GenomONE-CAb EX is a tool for efficient introduction
of IgG antibodies into
living cells using HVJ-Envelope (HVJ-E) vector for
the purpose of analysis
of the functions of cells and intracellular
HVJ Envelope (HVJ-E)?
Because antibodies cannot enter cells, past experiments using
antibodies focused primarily on extracellular
target molecules in living cells can be exposed to antibodies, it will be
possible to pursue new
dimensions of research related to cell function analysis, exploration
of target molecules for disease diagnosis
treatment, and so on.
Antibody Delivery Reagent is a
next-generation tool for antibody
introduction into cells which can meet following
Analysis of intracellular function
Screening of antibodies reacting to intracellular antigens
Advantages of intracellular antibody introduction (differences from existing knockout method)
With this kit, antibody can be incorporated into the
HVJ Envelope (HVJ-E), a transfection tool making use of
fusing ability of inactivated Sendai virus (HVJ: Hemagglutinating Virus of
Japan). If cells are
treated with HVJ-E including antibody, it will be
possible to achieve efficient introduction of IgG antibodies into
cytoplasm. This kit provides a totally new methodology for experiments,
overcoming the difficulties involved in
experiments using conventional
lipid-based reagents by which antibodies are introduced into cells by
With this system, the IgG antibody
incorporation enhancer, which was developed by our company,
the efficiency of inclusion
of IgG molecules into HVJ-E markedly compared to the existing
EX ). Thanks to this feature, this
system allows efficient introduction of
IgG molecules into the cytoplasm.
Papers related to
published by our
Y. et al.: Efficient delivery
of antibody into living cells using a novel HVJ envelope vector
J. Immunol. Methods, 332,10-17(2008).
Examples of antibody introduction into
delivery of anti-NPC
Actin filaments were stained
stained with phalloidin (red)
anti-NPC (nuclear pore complex
antibody, introduced into living
cells, moves onto
the nuclear membrane, holding its activity, where it binds specifically
to the antigen (resulting in
chromatic response of the nuclear membrane)
should be used as the fluorescence-labeled secondary antibody.
If whole antibody is used,
binding may take place, making it impossible for specific chromatic
responses to appear.
nuclear translocation of NF-κB
antibody Anti-NF-κB antibody
of anti-NF-κB antibody or anti-NPC antibody to (red
arrows) Raw 264.7 cells
inhibited nuclear translocation of NF-κB by LPS
stimulation (yellow arrows).
confocal laser scanning microscopy).
Inhibition of Nitric Oxide production
Introduction of anti-NF-kB antibodies or anti-NPC antibodies to
Raw 264.7 cells led to significant
inhibition of nitric oxide (NO) production.
(This experiment was performed as joint research with
Immuno-Biological Laboratories Co., Ltd.
We express our heartfelt thanks
to Dr. Kiyoshi Ishikawa and the other individuals involved.）
Reagent II (incorporation
(for suspension and
HVJ-E and Reagent I]
:Moisture can reduce the activities.
Refrigerated at 2-8°C,
sealed in an aluminum package.
and Buffer] : Refrigerated at 2-8°C.
suspension)] : Requires refrigerated storage (2-8°C)
and should be used
weeks. Since thawing of frozen suspension can reduce
the suspension should not be stored frozen.
For continuous use, store in a refrigerator (2-8°C)
for up to 2 weeks.
For extended storage,
freeze in working aliquots at -80°C
3 months. Thawing after freezing is possible
of each reagent]
HVJ-E]: The main frame of the vector into which the molecule to be
transferred is included.
It fuses with the cell membrane, allowing the
target molecule to be introduced into
I (antibody incorporation enhancer)]: A protein with high binding affinity
with HVJ-E and IgG
which increases incorporation efficiency of
antibody molecule into HVJ-E.
reagent)]: Increases permeability across the HVJ-E membrane and
incorporation of antibody.
A positively-charged peptide which increases affinity between
IgG-bearing HVJ-E (HVJ-E vector) and the cell (or tissue)
and thus increases the efficiency
Neutral buffer of physiological concentration used for suspending or
diluting HVJ-E or other purposes.
used with the method described in this package insert, the product can be
used for IgG antibody delivery as follows.
a 24-well plate
AB-001EX (HVJ-E:1 vial): 25
(HVJ-E:4 vials): 100
an 8-well chambered coverglass for fluorescence observation
introduction of DNA, siRNA and protein)
Cell fusion kit
[For preparation of hybridomas
(monoclonal antibodies) and research on development,
differentiation, and breeding (transplantation and replacement
Application data sheet (No.1)
data sheet No.2
Application data sheet No.3