For Laboratory Research Use Only. Not for Use in Clinical Diagnosis or Therapeutic Procedures.

 Product Name  Cat # Size Price
GenomONE-CF EX   CF001EX 1kit (HVJ-E 1vial set) distributor website

        

  GenomONE-CF EX   is a cell fusion kit composed of HVJ Envelope (HVJ-E) and special buffers.

  It can be used with both adhering cells and floating cells. Fusion of cells of the same
  or different types is possible with this kit in only 30 minutes.


  

     Cell fusion with GenomONE-CF EX   (HVJ-E Cell Fusion Kit) (time course of 67 minutes) BHK-21 cells labeled with red and
   green fluorescent dyes incubated at 37C in the presence of HVJ-E and observed under a confocal laser scanning
   microscope

   
What's HVJ Envelope (HVJ-E)?

 [Features] 
 This kit resolves the following problems, encountered when attempting cell fusion using 
  polyethylene glycol (PEG) or electrofusion


 
Operation is too complex

 Toxicity is high and the efficiency of cell fusion low

 Reproducibility is low

 Much time is needed to optimize fusion conditions

 Rapid or simultaneous processing of many samples is not possible due to the necessity 
   for strict control of reactions 

 [Principle] 
Principle for cell fusion triggered by HVJ-E

              

 

A local model of two cells cross-linked by one HVJ-E particle.

Step 1

If HVJ-E is added in amounts of more than several hundred HVJ-E per cell at low temperatures (0-8C), HVJ-E is immediately adsorbed on the cell surface mediated by the receptor (acetyl type sialic acid  recognized by HN protein) (Step 1), and cells undergo agglutination cross-linked by HVJ-E particles  (Step 2). At this stage, the hydrophobic domain at the N-terminal of cleaved F protein (F1) penetrates into the double lipid layer of the cell membrane, causing distortion of the membrane severe enough to allow an inflow of ions.
Step 2
Step 3
If this cell / HVJ-E complex is heated at 37C, the distortion of the cell membrane is further expanded, accompanied by temporary alteration of the cell membrane lining structure.  This change is transient  and the membrane soon returns to its normal structure.  However, if a strong hydrophobic connective  force is applied at this stage, fusion between cell membranes takes place (Step 3).

 [Reference] 
 Okada Y., et al., Exp. Cell Res., 93, 368-378 (1975).
 Okada, Y., Cell Fusion and Life Science, Brain Center (2001).

  [Applications] 

No. Reference Fused cell Application
1 Journal of Immunology, 173, 4297-4307 (2004). Dendritic ells and B16BL6 melanoma cells Cancer immunology
2 Mutation Research, 562, 11-17 (2004). Nornal human fibroblasts (G0/G1) and HeLa (M phase) Premature chromosome condensation (PCC) 
3 Radiation Research, 165, 59-67 (2006). Nornal human fibroblasts (G0/G1) and HeLa (M phase) Premature chromosome condensation (PCC) 
4 Chromosome Science, 9, 65-73 (2006). Microcell and HT1080
Microcell and hiMSC (immortalized human mesencymal stem cell)
Microcell mediated chromosome transfer 
5 Hybridoma (Larchmt), 26, 381-385 (2007). Rat lymph node cells (immunized) and mouse myeloma cells (P3U1) Preparation of monoclonal
[Immunogen: recombinant TARSH-CH protein]
6 Immunol. Lett., 121, 97-104 (2008). Rat lymph node cells (immunized) and mouse myeloma cells (P3U1) Preparation of monoclonal antibodies
7 Human Reproduction, 23, 1377-1384 (2008). Mouse enucleated oocyte and nucleus Nuclear transfer 
8 Biosystems, 92, 226-232 (2008). Rat cardiac myocute and  Rat cardiac fibroblast Analysis of cellular function
9 Journal of Cell Science,122, 912- 918 (2009). HeLa  Analysis of cellular function
10 Int. J. Oncol., 35, 249-255 (2009). Dendritic ells and Meth A cells (BALB/c-derived fibrosarcoma) Cancer immunology
11 Nature, 461, 367-372 (2009).  Macaca mulatta enucleated oocyte and nucleus Mitochondrial gene replacement
12 Reproduction, 137(4), 625-632 (2009). Mouse enucleated oocyte and nucleus Nuclear transfer 
13 Biochem. Biophys. Res. Commun., 390,    
 547-551 (2009).
Mouse spleen cells (immunized) and mouse myeloma cells (P3U1) Preparation of monoclonal antibodies
[Immunogen: synthetic human IDH1R132H peptide]
14 Biochem. Biophys. Res. Commun., 391,    
 750-755 (2010).
Mouse spleen cells (immunized) and mouse myeloma cells (P3U1) Preparation of monoclonal antibodies
[Immunogen: lacto-series gangliosides 30-isoLM1 and 30,60-isoLD1]
15 Stem Cells Dev., 19(2), 229-238 (2010). Mouse adult bone marrow mononuclear cells and Mouse Es cells
Mouse embryonic fibroblasts (from Oct4-GFP transgenic mice) and Mouse ES cells
Reprogramming of somatic cells
16 Biochem. Biophys. Res. Commun., 394,           1053-1057 (2010). Mouse embryonic fibroblasts and Mouse ES cells Reprogramming of somatic cells
17 Nature, 465, 8285 (2010). Human zygotes Pronuclear transfer
18 Nature Protocols, 5(6), 1138-1147 (2010).  Macaca mulatta enucleated oocyte and nucleus Mitochondrial gene replacement
19 Hybridoma (Larchmt), 29(1), 7-11 (2010). Rat lymph node cells (immunized) and mouse myeloma cells (P3U1) Preparation of monoclonal antibodies
20 Stem Cells. 28(8), 1338-1348 (2010). Human Es cells and Human fibroblasts Reprogramming of somatic cells
21 Vet. Immunol. Immunopathol., 138(3),              231-234 (2010). Mouse lymphoid cells (immunized) and mouse myeloma cells(P3X63Ag8.653) Preparation of monoclonal antibodies
[Immunogen: recombinant feline cystatin C]
22 Radiat. Res.,174(5), 566-573 (2010). Human fibroblasts  Premature chromosome condensation (PCC) 
23 FASEB J., 25(3), 830-839 (2011). Human adipose tissue-derived stem cells and Neonatal rat cardiomyocytes  Analysis of cellular function
24 Biochem. Biophys. Res. Commun., 406(4),    608-613 (2011).  Mouse spleen cells (immunized) and mouse myeloma cells (P3U1) Preparation of monoclonal antibodies
25 Biol. Reprod., 85, 70-77 (2011). Mouse enucleated oocyte and nucleolus-like body (NLB) GV transplantation

 [Specification]  

Cat. #

Freeze-dried HVJ-E*1(inactivated HVJ)
 

0.26mL/vial

(when reconstituted

HVJ-E suspending buffer
 

0.5mL/vial

Cell fusion buffer 

(20 X concentrate)*2
 

10mL/vial

CF001EX

1 vial

 1 vial

1 vial

 *1   HVJ-E: HVJ Envelope

      Moisture can reduce activity. Store the product in a refrigerator, sealed in an aluminum package.

 *2  Before use, dilute 1:20 with water (e.g.: endotoxin-free water for injection)

 [Frequency of use]
   For fusion of cells of the same or different types

   CF001EX (HVJ-E:1 vial) : 100 runs

  

   For preparing B cell hybridoma

   CF001EX (HVJ-E:1 vial) : 10 runs

      

 [Stability during storage]  

    [Quality assured period of freeze-dried HVJ-E] : See the aluminum package for HVJ-E. 
    [HVJ-E suspension prepared with HVJ-E suspending buffer]: 

             For continuous use, store in a refrigerator (2-8C) for up to two weeks.
             For extended storage, freeze in working aliquots at -80
C for up to 3 months. 
             Thawing after freezing is possible only once.
HVJ-E suspending buffer and Cell fusion Buffer should be stored 
             in a refrigerator (2-8
C).

 [Related Technical Information]  

  Instruction manual 

  Safety Information 

  Data sheet   Application (Nuclear Transplantation)
  Application (Hybridoma production)    FAQ
  Trouble shooting

  Reference list

  Movie (Experimental protocol for cell fusion)

 

 

[Related product

Antibody delivery reagent 

 (For introduction of antibody)

GenomONE-CAb EX

Transfection kit                                  

 (For introduction of DNA, siRNA and protein)

GenomONE-Neo EX

 

Order (distributor)

Contact us

Reference article

Related Technical Informations

 



Flyer

 



Instruction manual

 



Safety Information

 



Reference list

 



Data Sheet

 



Application
(Hybridoma production)

 



Application(Nuclear Transplantation)

 



FAQ

 



Trouble shooting
  

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