Insulin introduction to A549

Cell Information

Cell condition: 4.0~104cells/0.5mL/well of 24-well plate
1day culture, 50`80% confluency
Medium: DMEM+10%FBS

Protocol

Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: FITC-labeled insulin
Molecule conc.: 1g/L
Method: Method-1
Reagent volume: <Incorporation step>
HVJ-E: 20L
Insulin sol.: 5L
Reagent A: 5L
Buffer: ---
Reagent B: 3L
<Introduction step>
Reagent C: 2.5L
Buffer: 15L
Treatment/well: 8L/well of 24-well plate
Incubation time: 24hr

Result

Efficiency: @

GenomONE+insulin

insulin only

Reference: ---

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