BSA introduction to BHK-21

Cell Information

Cell condition: 2.5~104cells/0.5mL/well of 24-well plate
Medium: DMEM+10%FBS

Protocol

Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: Alexa-labeled BSA
Molecule conc.: 1g/L
Method: Method-1
Reagent volume: <Incorporation step>
HVJ-E: 20L
BSA sol.: 5L
Reagent A: 5L
Buffer: ---
Reagent B: 3L
<Introduction step>
Reagent C: 2.5L
Buffer: 15L
Treatment/well: 8L/well of 24-well plate
Incubation time: 24hr

Result

Efficiency: `100%

BSA+GenomONE

BSA only

.

Reference: ---

>>back