pEGFP introduction to FM3A

Cell Information

Cell condition: 2.5~105cells/0.5mL/well of 24-well plate
Medium: DMEM+10%FBS

Protocol

Reagent used: GenomONE
Trangient or Stable: Transient
Introduced molecule: pEGFP
Molecule conc.: 4g/L
Method: Method-2
Reagent volume: <Incorporation step>
HVJ-E: 20L
DNA/TE conc.: 5L
Reagent A: ---
Buffer: 5L
Reagent B: 1L
<Introduction step>
Reagent C: 2.5L
Buffer: 15L
Treatment/well(*): 8L/well of 24-well plate
(*)
Centrifuge 1500rpm~30min at 35 with well-plate.
After centrifuging, remove supernatant and add 0.5mL of medium on 1 well of 24-well plate.
Incubation time: 24hr

Result

Efficiency: 65%@

fluorecence

Phase contrast

Data from: Dr.Yasufumi Kaneda, Osaka University, Japan.

Reference: ---

>>back