Insulin introduction to FM3A

Cell Information

Cell condition: 2.5~105cells/0.5mL/well of 24-well plate
Medium: DMEM+10%FBS


Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: FITC-labeled insulin
Molecule conc.: 1g/L
Method: Method-1
Reagent volume: <Incorporation step>
HVJ-E: 20L
DNA/TE conc.: 5L
Reagent A: 5L
Buffer: ---
Reagent B: 3L
<Introduction step>
Reagent C: 2.5L
Buffer: 15L
Treatment/well(*): 8L/well of 24-well plate
Centrifuge 1500rpm~30min at 35 with well-plate.
After centrifuging, remove supernatant and add 0.5mL of medium on 1 well of 24-well plate.
Incubation time: 24hr


Efficiency: 99%


Phase contrast

Reference: ---