Rabbit IgG introduction to HL-60

Cell Information

Cell condition: 2.5~105cells/0.5mL medium
Medium: RPMI1640+10%FBS

Protocol

Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: Alexa-labeled Rabbit IgG
Molecule conc.: 1g/L
Method: Method-2 for suspension
Reagent volume: <Incorporation step>
HVJ-E: 20L
IgG sol. : 10L
Reagent A: ---
Buffer: 10L
Reagent B: 2L
<Introduction step>
Reagent C: 2.5L
Buffer: 32.5L
Treatment/well:(*) 8L/well of 24-well plate
(*)
Add 8L into 0.5mL of cell in micro test tube and centrifuge 12000rpm~30min at 4.
After centrifuging, remove supernatant and mix with 0.5mL of medium on 1 well of 24-well plate.
Incubation time: 24hr

Result

Efficiency: `100%@

fluorecence

Phase contrast

Data from: Dr.Akira Yuo, International Medical Center, Japan.

Reference: ---

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