pEGFP introduction to HUVEC

Cell Information

Cell condition: 2.5~104cells/0.5mL/well of 24-well plate 1day culture
Medium: EGM-2


Reagent used: GenomONE-Neo
Trangient or Stable: Transient
Introduced molecule: pEGFP
Molecule conc.: 4g/L
Method: Method-2
Reagent volume: <Incorporation step>
HVJ-E: 20L
DNA/TE sol.: 5L
Reagent A: ---
Buffer: 5L
Reagent B: 1L
<Introduction step>
Reagent C: 2.5L
Buffer: 15L
Treatment/well: 8L/well of 24-well plate
Incubation time: 24hr


Efficiency: 53%


Phase contrast

Data from: Dr.Yasufumi Kaneda, Osaka University, Japan.

Reference: ---