pEGFP introduction to K562

Cell Information

Cell condition: 1.0~106cells/2.0mL medium
Medium: RPMI1640+10%FBS

Protocol

Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: pEGFP
Molecule conc.: 4g/L
Method: Method-2 for suspension
Reagent volume: <Incorporation step>
HVJ-E: 40L
DNA/TE sol. : 10L
Reagent A: ---
Buffer: 10L
Reagent B: 2L
<Introduction step>
Reagent C: 5L
Buffer: 35L
Treatment/well:(*) 35L/well of 6-well plate
(*)
Add 35L into 1.0~106cells/0.5mL medium in micro test tube and centrifuge 10000g~30min at 35.
After centrifuging, remove supernatant and mix with 2.0mL of medium on 1 well of 6-well plate.
Incubation time: 24hr

Result

Efficiency: N.D

fluorescence

Phase contrast

Data from: Yasufumi Kaneda, Osaka university, Japan..

Reference: ---

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