-galactosidase introduction to NIH3T3

Cell Information

Cell condition: 1.0~105cells/2.0mL/well of 6-well plate
Medium: DMEM+10%FBS

Protocol

Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: -galactosidase
Molecule conc.: 1g/L
Method: Method-1
Reagent volume: <Incorporation step>
HVJ-E: 40L
-gal sol. 10L
Reagent A: 10L
Buffer: ---
Reagent B: 6L
<Introduction step>
Reagent C: 5L
Buffer: 30L
Treatment/well: 35L/well of 6-well plate
Incubation time: 24hr

Result

Efficiency: ---@

X-gal staining

Reference: ---

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