BSA introduction to PC-12

Cell Information

Cell condition: 2.5~104cells/0.5mL/well of 24-well plate
differentiate into sympathetic-like neurons following stimulation with NGF
Medium: DMEM+10%FBS

Protocol

Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: Alexa-labeled BSA
Molecule conc.: 1g/L
Method: Method-1
Reagent volume: <Incorporation step>
HVJ-E: 20L
DNA/TE sol.: 5L
Reagent A: 5L
Buffer: ---
Reagent B: 3L
<Introduction step>
Reagent C: 2.5L
Buffer: 15L
Treatment/well(*): 8L/well of 24-well plate
(*)
Centrifuge 3000rpm~10min at 35 with well-plate.
After centrifuging, remove supernatant and add 0.5mL of medium on 1 well of 24-well plate.
Incubation time: 24hr

Result

Efficiency: 77%@

fluorescence

Phase contrast

Reference: ---

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