siRNA introduction to Rat primary cardiac cell

Cell Information

Cell condition: 70`90% confluency
Medium: @

Protocol

Reagent used: GenomONE-Neo
Trangient or Stable: Transient
Introduced molecule: Cy3-labeled siRNA
Molecule conc.: 0.28g/L
Method: Method-2
Reagent volume: <Incorporation step>
HVJ-E: 20L
siRNA sol.: 15L
Reagent A: ---
Buffer: ---
Reagent B: 1.5L
<Introduction step>
Reagent C: 2.5L
Buffer: 32.5L
Treatment/well: 35L/2.0mL of cell/well of 6-well plate
Incubation time: 24hr

Result

Efficiency: ---

fluorecence

Phase contrast

Data from: M. Arai, A. Watanabe, Gunma University, Japan

Reference: ---

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