BSA introduction to RAW264.7

Cell Information

Cell condition: 5.0~104cells/0.5mL/well of 24-well plate
1day culture, 50`80% confluency
Medium: DMEM+10%FBS


Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: Alexa-labeled BSA
Molecule conc.: 1g/L
Method: Method-1
Reagent volume: <Incorporation step>
HVJ-E: 20L
ODN sol.: 5L
Reagent A: 5L
Buffer: ---
Reagent B: 3L
<Introduction step>
Reagent C: 2.5L
Buffer: 15L
Treatment/well: 8L/well of 24-well plate
Incubation time: 24hr


Efficiency: 64%@
Fluorescent Phase Contrast

Reference: ---