ODN introduction to U937

Cell Information

Cell condition: 2.5~105cells/0.5mL medium
Medium: @


Reagent used: GenomONE
Trangient or Stable: ---
Introduced molecule: FITC-labeled s-oligo(15mer)
Molecule conc.: 1g/L
Method: Method-2 for suspension
Reagent volume: <Incorporation step>
HVJ-E: 20L
ODN sol.: 10L
Reagent A: ---
Buffer: 10L
Reagent B: 2L
<Introduction step>
Reagent C: 2.5L
Buffer: 32.5L
Treatment/well(*): 8L/well of 24-well plate
Add 8L into 2.5~105cells/0.5mL medium in micro test tube and centrifuge 10000g~10min at 4.
After centrifuging, remove supernatant and mix with 0.5mL of medium on 1 well of 24-well plate.
ncubation time: 24hr


Efficiency: `100%


Phase contrast

Data from: Dr. Akira Yuo, International Medical Center, Japan..

Reference: ---