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Cas9 protein/gRNA transfection reagent
GenomONE TM-GE

Product name Cat. # Specification Frequency of use
(96-well plate)
Price excluding tax Price including tax
Freeze-dried HVJ-E Reagent F,G Buffer
GenomONE-GE GG001 1 vial each 1 vial 1 vial 325 ¥28,000 ¥30,800
GG004 4 vials each 1 vial 1 vial 1,300 ¥75,000 ¥82,500
GG016 16 vials each 4 vials 2 vials 5,200 ¥280,000 ¥308,000

GenomONE-GE enables efficient Cas9 protein/gRNA transfection in a variety of difficult-to-transfect cells.
In case of plasmid DNA transfection for Cas9 and gRNA expression, please use GenomONE-GX .

*This product is sold for research purpose only. It may not be used for treatment or other clinical purposes or for intra- and extracorporeal diagnosis in humans or animals.

Features of GenomONE-GE

  • Applicable for CRISPR-Cas9-mediated knock-out and knock-in
  • Applicable for difficult-to-transfect cells (e.g., Mouse primary T cell, U-937, Jurkat)
  • Simple operability (completion of transfection within 10 min)

Cas9 protein and PPIB-targeting gRNA transfection in mouse primary T cell


Cleaved efficiency (%) = sum of cleaved band intensities/(sum of the cleaved and parental band intensities) x100

One day after stimulation of mouse splenocytes with PMA/ionomycin, T cells were isolated. Cas9 protein and PPIB-targeting gRNA were then transfected using GenomONE-GE, product C, or product T (competitors). Two days after transfection, genome editing efficiency was evaluated using T7 endonuclease I mismatch cleavage assay.

Cas9 protein, gRNA, donor DNA transfection (knock-in)


Knock-in efficiency (%) = sum of band intensities cleaved with BamHI enzyme / (band intensity cut with BamHI enzyme and total unbroken band intensity by BamHI enzyme)x100

Cas9 protein, gRNA and donor DNA (ssODN) were transfected into HeLa cells using GenomONE-GE. A donor DNA was designed to carry the recognition site of BamHI restriction enzyme. Two days after transfection, knock-in efficiency was evaluated using RFLP.

Cas9 protein and PPIB-targeting gRNA transfection in HeLa and U-937 cells


Cleaved efficiency (%) = sum of cleaved band intensities/(sum of the cleaved and parental band intensities) x100

Cas9 protein and PPIB-targeting gRNA were transfected into HeLa and U-937 cells using GenomONE-GE, product C, or product T (competitors). Two days after transfection, genome editing efficiency was evaluated using T7 endonuclease I mismatch cleavage assay.

Related technical information

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