Gene Transfection Reagent
|Product name||Cat. #||Specification||Price
|GenomONE-GX||GX001||Reagent 1～5, Enhancer (Each 1 vial)||¥11,000||¥12,100|
|GX004||Reagent 1～5, Enhancer (Each 4 vials)||¥40,000||¥44,000|
|GX016||Reagent 1～5, Enhancer (Each 16 vials)||¥150,000||¥165,000|
|GX040||Reagent 1～5, Enhancer (Each 40 vials)||¥310,000||¥341,000|
GenomONE-GX is a kit for dedicated use for transfection of DNA using the lipid-like substance and auxiliary reagents (including HVJ-E and Enhancer). In difficult-to-transfect cells (e.g. Jurkat, K-562, RAW 264.7 and THP-1), GenomONE-GX demonstrates higher transfection performance compared to representative lipofection reagent.
[HVJ-E : Hemagglutinating virus of Japan （HVJ） Envelope ; inactivated Sendai Virus]
*This product is sold for research purpose only. It may not be used for treatment or other clinical purposes or for intra- and extracorporeal diagnosis in humans or animals.
Features of GenomONE-GX
- A novel gene delivery vector composed of a lipid-like substance and HVJ-E.
- GenomONE-GX 's Enhancer suppresses innate immune responses by plasmid DNA and improves exogenous gene expression.
- Exogenous gene expression is enhanced by GenomONE-GX using a combination of KALA peptides.
To protect themselves from exogenous factors such as viruses, cells have an intracellular defense system that recognizes exogenous DNA. In cells with transgene expression intrinsically suppressed by innate immune signaling, GenomONE-GX 's Enhancer can be expected to increase transgene expression levels, as it inhibits innate immune signaling in response to the recognition of exogenous DNA. KALA peptides are able to destabilize the endosomal membrane and enhance the transfection efficiency of non-viral gene delivery vectors.
Various cell lines were transfected with a reporter plasmid encoding TurboGFP under a CAG promoter using either GenomONE-GX or Product L (a competitor) following the manufacturer’s instructions. One day or two days after transfection, TurboGFP expression was detected by fluorescence microscopy.
KALA peptide-mediated enhancement of transgene expression
Two days after transfection, TurboGFP expression was detected by fluorescence microscopy. GenomONE-GX with the addition of KALA peptide exhibited higher transfection efficiency than that of Product L (a competitor). KALA peptide also upregulated transgene expression in HeLa S3, Hep G2, Hs68, HuH-7, NIH/3T3, U-937, CHO-K1, L929, and P3X63Ag8.653 cells.
※KALA peptide is not included in the GenomONE-GX kit. Please prepare the KALA peptide solution in advance.
Screening of cells expressing the gene of interest by means of a drug resistance gene
RAW 264.7 cells were seeded in a 24-well plate on the day before transfection, and Jurkat cells were seeded in a 12-well plate on the day of transfection. Transfection was performed with a plasmid containing TurboGFP and a neomycin-resistant gene using GenomONE-GX, and on the following day, the medium was exchanged for fresh medium containing G418 for further culture. RAW 264.7 cells were subjected to fluorescence microscopy 6 days after transfection (Day 6). Jurkat cells were transferred to a 48-well plate on the day following transfection and then to a 96-well plate on Day 4 to avoid extremely low cell density. The cells were then subjected to fluorescence microscopy on Day 8.
Various cell lines were transfected with a reporter plasmid encoding luciferase under a CAG promoter using either GenomONE-GX or Product L (a competitor) following manufacturer instructions. One day after transfection, luciferase expression was measured.
Validation of optimal transfection conditions
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