Products

Research Reagents

Gene, siRNA/miRNA, protein transfection reagent (in vivo)
GenomONE TM-Neo EX

GenomONE-Neo EX is a novel and unique transfection kit which employ the membrane fusion ability of the envelope of Sendai virus(Hemagglutinating virus of Japan: HVJ). HVJ Envelope (HVJ-E) is a nonproliferative and noninfectious vesicle purified after complete inactivation of Sendai virus genomic RNA. HVJ-E can introduce a variety of molecules (plasmid DNA, siRNA/miRNA, protein) into cells via membrane fusion.

*This product is sold for research purpose only. It may not be used for treatment or other clinical purposes or for intra- and extracorporeal diagnosis in humans or animals.

Features

  • HVJ-E can incorporate various molecules (plasmid DNA, siRNA/miRNA, proteins).
  • HVJ-E-incorporated molecules are introduced into cells via membrane fusion.
  • HVJ-E is also applicable to in vivo transfection.

Specification

Product name Cat. # Specification
Store at 4℃
Freeze-dried HVJ-E Reagent A,B,C,Buffer
GenomONE-Neo EX GN001EX 1 vial Each 1 vial
GN004EX 4 vials Each 1 vial
GN016EX 16 vials Each 4 vials
GN040EX 40 vials Each 10 vials

Applications (in vivo)

Title Target cell/Tissues Introduced molecule
Rat lumbar dorsal horn siRNA
Rat submandibular gland

Excerpt from "Intrathecal administration of siRNA to rats"

Immunnohistochemical staining (dorsal horn)

By using HVJ-E vector, NK1R siRNA was administered into the spinal cord. Immunohistochemical reaction of NK1R was suppressed by siRNA-treated during 1 to 7 days.

【Data】
  • Dr. Rumi Naono-Nakayama
    Division of Anatomy and Cell Biology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University (Japan)
  • Dr. Toshikazu Nishimori
    Department of Psychiatry, Faculty of Medicine, University of Miyazaki (Japan)
【Related article】
Eur. J. Pharmacol., 670, 448-457 (2011).

Applications (in vitro)

Title Target cell/Tissues Introduced molecule
Neonatal rat myocardial cells (primary cultures) siRNA
U937

Intracellular delivery of Cre recombinase (protein)

By introducing Cre recombinase (protein) into 2-2 cells with HVJ Envelope, loxp sites inserted in the genome sequence were deleted and Lac Z gene expression was induced.

2-2 (monkey, African green, RIKEN BioResource Center): 35 copies of PCAG-loxP-neopA-loxP-LacZ were tandemly inserted into the genome.

Introduction of Bin-1 siRNA (C2C12)

Twenty-four hours after induction of differentiation, C2C12 cells (mouse myoblast cell line) were transfected with Bin-1 siRNA using conventional representative siRNA transfection reagents (products A, B, and C)and GenomONE-Neo. Other products failed to exert sufficient knock-down effects, demonstrating the superiority of GenomONE-Neo to these products.

【Data】
  • Dr. Chie Kojima
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University (Japan)
  • Dr. Hisataka Sabe
    Department of Molecular Biology, Osaka Bioscience Institute (Japan)
【Related article】
C. Kojima et al. EMBO Journal, 23, 4413-4422 (2004).

Inquiry Contacts about Research Reagents

  • Business Development Division, Healthcare Business Headquarters

  • TEL:+81-6-6444-7182

  • Inquiry form